other:Article Title: Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons
Article Snippet: Primary antibodies: rabbit anti-Lhx3 (1:2000, Abcam Cat# ab14555, RRID:AB_301332); rabbit anti-Foxp1(1:20000, Abcam Cat# ab16645, RRID:AB_732428); goat anti-Foxp1(1:100 R&D systems Cat# AF4534, RRID:AB_2107102); rabbit anti-Hoxc8 (1:5000 SigmaAldrich Cat# HPA028911, RRID:AB_10602236); sheep anti-GFP (1:1000 AbD Serotec/Bio-Rad Cat# 4745–1051, RRID:AB_619712); rabbit anti-RFP (1:500 Abcam Cat# ab62341, RRID: AB_945213); mouse anti-MNR2/MNX1/HB9 (1:50 DSHB Cat# 81.5C10, RRI D:AB_2145209); goat anti-Chat (1:100 Millipore/Sigma Cat# ab144P, RRID:AB_2079751); mouse anti-COUP-TF2/NR2F2 (1:200 R&D systems Cat# PP-H7147-00, RRID:AB_2155627); mouse anti-COUP-TF1/NR2F1 (1:500 R&D systems Cat# PP-H8132-00, RRID:AB_2155494); rabbit anti-Evi1/Mecom (1:500 Cell Signaling Technology Cat# 2593, RRID:AB_2184098); goat anti-Isl1 (1:1000 Neuromics Cat# GT15051, RRID:AB_2126323); rabbit anti-Satb2 (1:1000 Abcam Cat# ab92446, RRID:AB_10563678); guinea pig antiSatb2 (1:1000 Synaptic Systems Cat# 327004, RRID:AB_2620070); rat anti-Bcl11b/Ctip2 (1:1000 Abcam Cat# ab18465, RRID:AB_2064130); rabbit anti-Zfhx4 (1:200 Novus Cat# NBP1-82156, RRID:AB_11020060); rabbit anti-Calbindin D-28K/Calb1 (1:1000 Millipore/Sigma Cat# AB1778, RRID:AB_2068336); rabbit anti-Nfib (1:1000 Novus Cat# NBP1-81000, RRID:AB_11027763); rabbit antiGrm5 (1:500 Millipore/Sigma Cat# AB5675, RRID:AB_2295173); sheep anti-Ebf2 (1:500 Novus Cat# AF7006, RRID:AB_10972102); rabbit anti-Nrp2 (1:200 Cell Signaling Technology Cat# 3366, RRID:AB_2155250).
Article Title: Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons
Article Snippet: Primary antibodies used were: rabbit anti-Lhx3 (1:2000, Abcam Cat# ab14555, RRID:AB_301332); rabbit anti-Foxp1(1:20000, Abcam Cat# ab16645, RRID:AB_732428); goat anti-Foxp1(1:100 R&D systems Cat# AF4534, RRID:AB_2107102); rabbit anti-Hoxc8 (1:5000 Sigma-Aldrich Cat# HPA028911, RRID:AB_10602236); sheep anti-GFP (1:1000 AbD Serotec/Bio-Rad Cat# 4745–1051, RRID:AB_619712); rabbit anti-RFP (1:500 Abcam Cat# ab62341, RRID: AB_945213); mouse anti-MNR2/MNX1/HB9 (1:50 DSHB Cat# 81.5C10, RRID:AB_2145209); goat anti-Chat (1:100 Millipore/Sigma Cat# ab144P, RRID:AB_2079751); mouse anti-COUP-TF2/NR2F2 (1:200 R&D systems Cat# PP-H7147-00, RRID:AB_2155627); mouse anti-COUP-TF1/NR2F1 (1:500 R&D systems Cat# PP-H8132-00, RRID:AB_2155494); rabbit anti-Evi1/Mecom (1:500 Cell Signaling Technology Cat# 2593, RRID:AB_2184098); goat anti-Isl1 (1:1000 Neuromics Cat# GT15051, RRID:AB_2126323); rabbit anti-Satb2 (1:1000 Abcam Cat# ab92446, RRID:AB_10563678); guinea pig anti-Satb2 (1:1000 Synaptic Systems Cat# 327004, RRID:AB_2620070); rat anti-Bcl11b/Ctip2 (1:1000 Abcam Cat# ab18465, RRID:AB_2064130); rabbit anti-Zfhx4 (1:200 Novus Cat# NBP1-82156, RRID:AB_11020060); rabbit anti-Calbindin D-28K/Calb1 (1:1000 Millipore/Sigma Cat# AB1778, RRID:AB_2068336); rabbit anti-Nfib (1:1000 Novus Cat# NBP1-81000, RRID:AB_11027763); rabbit anti-Grm5 (1:500 Millipore/Sigma Cat# AB5675, RRID:AB_2295173); sheep anti-Ebf2 (1:500 Novus Cat# AF7006, RRID:AB_10972102); rabbit anti-Nrp2 (1:200 Cell Signaling Technology Cat# 3366, RRID:AB_2155250).
Article Title: Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons.
Article Snippet: Primary antibodies used were: rabbit anti-Lhx3 (1:2000, Abcam Cat# ab14555, RRID:AB_301332); rabbit anti-Foxp1(1:20000, Abcam Cat# ab16645, RRID:AB_732428); goat anti-Foxp1(1:100 R&D systems Cat# AF4534, RRID:AB_2107102); rabbit anti-Hoxc8 (1:5000 Sigma-Aldrich Cat# HPA028911, RRID:AB_10602236); sheep anti-GFP (1:1000 AbD Serotec/ Bio-Rad Cat# 4745–1051, RRID:AB_619712); rabbit anti-RFP (1:500 Abcam Cat# ab62341, RRID: AB_945213); mouse anti-MNR2/MNX1/HB9 (1:50 DSHB Cat# 81.5C10, RRID:AB_2145209); goat anti-Chat (1:100 Millipore/Sigma Cat# ab144P, RRID:AB_2079751); mouse anti-COUPTF2/NR2F2 (1:200 R&D systems Cat# PP-H7147-00, RRID:AB_2155627); mouse anti-COUP-TF1/NR2F1 (1:500 R&D systems Cat# PP-H8132-00, RRID:AB_2155494); rabbit anti-Evi1/Mecom (1:500 Cell Signaling Technology Cat# 2593, RRID:AB_2184098); goat anti-Isl1 (1:1000 Neuromics Cat#GT15051, RRID:AB_2126323); rabbit anti-Satb2 (1:1000AbcamCat# ab92446, RRID:AB_10563678); guinea pig anti-Satb2 (1:1000 Synaptic SystemsCat#327004, RRID:AB_2620070); rat anti-Bcl11b/Ctip2 (1:1000 Abcam Cat# ab18465, RRID:AB_2064130); rabbit anti-Zfhx4 (1:200 Novus Cat# NBP1-82156, RRID:AB_11020060); rabbit anti-Calbindin D28K/Calb1 (1:1000 Millipore/Sigma Cat# AB1778, RRID:AB_2068336); rabbit anti-Nfib (1:1000 Novus Cat# NBP1-81000, RRID:AB_11027763); rabbit anti-Grm5 (1:500 Millipore/Sigma Cat# AB5675, RRID:AB_2295173); sheep anti-Ebf2 (1:500 Novus Cat# AF7006, RRID:AB_10972102); rabbit anti-Nrp2 (1:200 Cell Signaling Technology Cat# 3366, RRID:AB_2155250).
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